Author: Dr. S. B. Gurumurthy, Dr. K. Kumutha
Outlines of Introductory Microbiology
- Milestones in the field of Microbiology
- Scope and Importance
- The Microscopic examination of microorganisms
- Microbiological stains
- 7. Structure and organization of Microorganisms
- Types of Culture Media and Preculture Techniques
- 10. Microbial Nutrition
- Microbial Growth
- 13. Viruses
14, 15. Microbial Genetics
- 18. Interrelationship between microorganisms
1-2. Study of compound microscope/bright field microscope
- Study of parts of microscope
- Examination of animate and inanimate objects
- Working of microscope
- Microscopic examination of living microorganisms by Temporary Wet Mount (TWM) technique.
- Hanging drop technique for demonstrating motility of living bacteria.
- Simple Staining technique
- Differential staining technique-Gram stain
- Study of structural staining technique
- Sterilization techniques
- Preparation of culture media (nutrient agar)
- Preparation of nutrient broth
- Preparation of nutrient agar slants/slopes
12-13. Isolation and culture of microorganisms by streak plate method
14-15. Isolation and culture of microorganisms by serial dilution technique and pour-plate method
17-18. Determination of rate of microbial growth by Turbidometric technique.
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