Principle:
This test is based on the principle that methyl blue (an oxidation-reduction dye or indicator) which is blue in its oxidized state, is reduced to colorless compound (leuco form) as a result of the metabolic activities of bacteria in milk. When a solution of dye is added, the organisms present in milk consume the dissolved oxygen and lower O-R potential to a level where methyl blue or similar indicators are reduced or decolorized.
Reagent Required:
Methylene Blue Reduction Dye
MBRT Procedure:
MBRT Test result and interpretations:
MBRT TEST RESULTS | 5 hrs and above | Very Good |
3 – 4 hrs | Good | |
1 – 2 hrs | Fair | |
Half an hour | Poor |
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Does this experiment distinguish between beneficial bacteria and those which are contaminants
Enjoyed reading through this, very good stuff, thankyou .